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gna galanthus nivalis vector lab l 1240 gsl  (Vector Laboratories)


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    Vector Laboratories gna galanthus nivalis vector lab l 1240 gsl
    Gna Galanthus Nivalis Vector Lab L 1240 Gsl, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/L-1240-5/Unconjugated+Galanthus+Nivalis+Lectin+(GNL)/pm21133419__pr1010873_si_001-0-0-3
    Average 95 stars, based on 217 article reviews
    gna galanthus nivalis vector lab l 1240 gsl - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Purification:

    Article Title: Diverse priming outcomes under conditions of very rare precursor B cells
    Article Snippet: .. The trimer was co-transfected with furin into 293F cells and purified using Galanthus nivalis lectin affinity chromatography (Vector Laboratories) followed by SEC purification using Superdex 200 16/600 PG column (Cytiva). .. Trimers that were used as SPR reagents were expressed with C-terminal 6xHis-tag and purified using a HIS-TRAP column followed by size exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column (Cytiva) as described previously .

    Article Title: Immunofocusing on the conserved fusion peptide of HIV envelope glycoprotein in rhesus macaques
    Article Snippet: .. Six days after transfection, the cell supernatants were harvested by centrifugation and purified using Galanthus nivalis lectin (Vector Laboratories) affinity chromatography followed by size exclusion chromatography (SEC) using a HiLoad 16/600 Superdex 200 pg (Cytiva) column. .. Further purification was performed using two rounds of negative selection columns produced using CNBr-activated Sepharose 4B beads (Cytiva) coupled to non-neutralizing mAbs according to the manufacturer’s instructions.

    Affinity Chromatography:

    Article Title: Diverse priming outcomes under conditions of very rare precursor B cells
    Article Snippet: .. The trimer was co-transfected with furin into 293F cells and purified using Galanthus nivalis lectin affinity chromatography (Vector Laboratories) followed by SEC purification using Superdex 200 16/600 PG column (Cytiva). .. Trimers that were used as SPR reagents were expressed with C-terminal 6xHis-tag and purified using a HIS-TRAP column followed by size exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column (Cytiva) as described previously .

    Article Title: Immunofocusing on the conserved fusion peptide of HIV envelope glycoprotein in rhesus macaques
    Article Snippet: .. Six days after transfection, the cell supernatants were harvested by centrifugation and purified using Galanthus nivalis lectin (Vector Laboratories) affinity chromatography followed by size exclusion chromatography (SEC) using a HiLoad 16/600 Superdex 200 pg (Cytiva) column. .. Further purification was performed using two rounds of negative selection columns produced using CNBr-activated Sepharose 4B beads (Cytiva) coupled to non-neutralizing mAbs according to the manufacturer’s instructions.

    other:

    Article Title: Comparison of the Differing Impacts of Lowered N-Acetylglucosaminyltransferase-Ia/b Activity on Motor and Sensory Function in Zebrafish
    Article Snippet: Post migration, gels were either stained with Coomassie Brilliant Blue (MP Biomedical, Solon, OH, USA) or utilized to transfer proteins to a PVDF membrane (Whatman, Dassel, Germany) for lectin blotting, following previously established protocols [ ].

    Transfection:

    Article Title: Immunofocusing on the conserved fusion peptide of HIV envelope glycoprotein in rhesus macaques
    Article Snippet: .. Six days after transfection, the cell supernatants were harvested by centrifugation and purified using Galanthus nivalis lectin (Vector Laboratories) affinity chromatography followed by size exclusion chromatography (SEC) using a HiLoad 16/600 Superdex 200 pg (Cytiva) column. .. Further purification was performed using two rounds of negative selection columns produced using CNBr-activated Sepharose 4B beads (Cytiva) coupled to non-neutralizing mAbs according to the manufacturer’s instructions.

    Centrifugation:

    Article Title: Immunofocusing on the conserved fusion peptide of HIV envelope glycoprotein in rhesus macaques
    Article Snippet: .. Six days after transfection, the cell supernatants were harvested by centrifugation and purified using Galanthus nivalis lectin (Vector Laboratories) affinity chromatography followed by size exclusion chromatography (SEC) using a HiLoad 16/600 Superdex 200 pg (Cytiva) column. .. Further purification was performed using two rounds of negative selection columns produced using CNBr-activated Sepharose 4B beads (Cytiva) coupled to non-neutralizing mAbs according to the manufacturer’s instructions.

    Size-exclusion Chromatography:

    Article Title: Immunofocusing on the conserved fusion peptide of HIV envelope glycoprotein in rhesus macaques
    Article Snippet: .. Six days after transfection, the cell supernatants were harvested by centrifugation and purified using Galanthus nivalis lectin (Vector Laboratories) affinity chromatography followed by size exclusion chromatography (SEC) using a HiLoad 16/600 Superdex 200 pg (Cytiva) column. .. Further purification was performed using two rounds of negative selection columns produced using CNBr-activated Sepharose 4B beads (Cytiva) coupled to non-neutralizing mAbs according to the manufacturer’s instructions.

    Whole Genome Amplification:

    Article Title: Supplementary Figure 1. Scan images of the TSA lectin microarray for LNCAP PSA (A) and LNCAP PSA treated with α1-2 fucosidase (B). The red box indicates the location of UEA I on the microarray
    Article Snippet: .. GNA Galanthus nivalis Vector Lab L-1240 GSL-I B4 Griffonia Simplicifolia -TB4 Vector Lab L-1104 PTL-I Psophocarpus tetragonolobus Vector Lab L-1360 BSL-II Bandeiraea simplicifolia II Vector Lab L-1210 VVA Vicia villosa Vector Lab L-1230 ECA Erythrina cristagalli Vector Lab L-1140 HHL Hippeastrum Hybrid Vector Lab L-1380 BPL Bauhinia purpurea Vector Lab L-1280 AAL Aleuria aurantia Vector Lab L-1390 ConA Canavalia ensiformis Vector Lab DBA Dolichos biflorus Vector Lab PNA Arachis hypogaea Vector Lab SBA Glycine max Vector Lab UEA Ulex europaeus Vector Lab WGA Triticum aestivum Vector Lab RCA120 Ricinus communis Vector Lab BSL-I Bandeiraea simplicifolia I Vector Lab PEA Pisum sativum Vector Lab PHA-E Phaseolus vulgaris E Vector Lab PHA-L Phaseolus vulgaris L Vector Lab SJA Sophora japonica Vector Lab S-WGA Succinylated Triticum aestivum Vector Lab SNA I Sambucus nigra EY lab L-6802 Jacalin Artocarpas integliforia EY lab L-6301 Calsepa Calystegia sepium EY lab L-8011 LcHA Lens culinaris EY lab L-1402 MPA Maclura pomifera EY lab L-3901 MNA-G Morus nigra G EY lab L-9005 NPA Narcissus pseudonarcissus EY lab L-8006 STA Solanum tuberosum EY lab L-4701 MAA Maackia amurensis EY lab L-7801 DSA Datura stramonium EY lab L-5701 LTL Lotus tetragonolobus EY lab L-1601 SSA Sambucus sieboldiana EY lab L-3501 PWM, PWA Phytolacca americana EY lab L-1901 UDA Urtica dioica EY lab L-8005 ABA Agaricus bisporus EY lab L-5001 WFA Wisteria floribunda EY lab L-3101 LK-2000 ..

    Article Title: Supplementary Figure 1. Scan images of the TSA lectin microarray for LNCAP PSA (A) and LNCAP PSA treated with α1-2 fucosidase (B). The red box indicates the location of UEA I on the microarray
    Article Snippet: .. GNA Galanthus nivalis Vector Lab L-1240 GSL-I B4 Griffonia Simplicifolia -TB4 Vector Lab L-1104 PTL-I Psophocarpus tetragonolobus Vector Lab L-1360 BSL-II Bandeiraea simplicifolia II Vector Lab L-1210 VVA Vicia villosa Vector Lab L-1230 ECA Erythrina cristagalli Vector Lab L-1140 HHL Hippeastrum Hybrid Vector Lab L-1380 BPL Bauhinia purpurea Vector Lab L-1280 AAL Aleuria aurantia Vector Lab L-1390 ConA Canavalia ensiformis Vector Lab DBA Dolichos biflorus Vector Lab PNA Arachis hypogaea Vector Lab SBA Glycine max Vector Lab UEA Ulex europaeus Vector Lab WGA Triticum aestivum Vector Lab RCA120 Ricinus communis Vector Lab BSL-I Bandeiraea simplicifolia I Vector Lab PEA Pisum sativum Vector Lab PHA-E Phaseolus vulgaris E Vector Lab PHA-L Phaseolus vulgaris L Vector Lab SJA Sophora japonica Vector Lab S-WGA Succinylated Triticum aestivum Vector Lab SNA I Sambucus nigra EY lab L-6802 Jacalin Artocarpas integliforia EY lab L-6301 Calsepa Calystegia sepium EY lab L-8011 LcHA Lens culinaris EY lab L-1402 MPA Maclura pomifera EY lab L-3901 MNA-G Morus nigra G EY lab L-9005 NPA Narcissus pseudonarcissus EY lab L-8006 STA Solanum tuberosum EY lab L-4701 MAA Maackia amurensis EY lab L-7801 DSA Datura stramonium EY lab L-5701 LTL Lotus tetragonolobus EY lab L-1601 SSA Sambucus sieboldiana EY lab L-3501 PWM, PWA Phytolacca americana EY lab L-1901 UDA Urtica dioica EY lab L-8005 ABA Agaricus bisporus EY lab L-5001 WFA Wisteria floribunda EY lab L-3101 LK-2000 LK-3000 Supplementary Figure 1. ..

    Enzyme-linked Immunospot:

    Article Title: The saponin monophosphoryl lipid A nanoparticle adjuvant induces dose-dependent HIV vaccine responses in non-human primates
    Article Snippet: .. Absolute ID50 values were calculated using 574 normalized relative luminescence units and a customized nonlinear regression model with the bottom 575 constraint set to 0 and top constraint set to the <100: 576 = Bottom+ Top − Bottom 1 + 10(./0123/456789:; < =)∗@A44 B4/C7 D ./0E F/CELISPOT 579 27 Bone marrow aspirates were processed to harvest mononuclear cells which were serially diluted and plated 580 onto pre-coated wells with BG505 MD39 antigen (bound via Galanthus Nivalis Lectin [Vector laboratories 581 Cat# L-1240]) or total goat anti-human Ig antibody (Southern Biotech Cat# 2010-01) as captures. ..



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    Increased levels of oligomannose-type N-glycans in adult mgat1a −/− zebrafish. Lectin blots of tissue homogenates from mgat1a+/+ , mgat1a −/− and mgat1a+/ − fish, as indicated ( A – F ). Electrophoresed proteins were probed with GNL (left panels). Protein gels stained with Coomassie blue (CB) (right panels) to demonstrate equal protein loads among the samples. Quantification of the oligomannosylated proteins from swim bladder (SB, n = 3), skin (S, n = 3), heart (H, n = 3), skeletal muscle (SM, n = 3), spinal cord (SC, n = 3), and brain (B, n = 4) ( G ). Numbers adjacent to the blots denote protein markers (in kDa). Data is mean ± S.E. and compared via Student’s t -test (* p at 0.05).

    Journal: International journal of translational medicine (Basel, Switzerland)

    Article Title: Comparison of the Differing Impacts of Lowered N-Acetylglucosaminyltransferase-Ia/b Activity on Motor and Sensory Function in Zebrafish

    doi: 10.3390/ijtm5030036

    Figure Lengend Snippet: Increased levels of oligomannose-type N-glycans in adult mgat1a −/− zebrafish. Lectin blots of tissue homogenates from mgat1a+/+ , mgat1a −/− and mgat1a+/ − fish, as indicated ( A – F ). Electrophoresed proteins were probed with GNL (left panels). Protein gels stained with Coomassie blue (CB) (right panels) to demonstrate equal protein loads among the samples. Quantification of the oligomannosylated proteins from swim bladder (SB, n = 3), skin (S, n = 3), heart (H, n = 3), skeletal muscle (SM, n = 3), spinal cord (SC, n = 3), and brain (B, n = 4) ( G ). Numbers adjacent to the blots denote protein markers (in kDa). Data is mean ± S.E. and compared via Student’s t -test (* p at 0.05).

    Article Snippet: The transferred proteins were then probed with Galanthus nivalis lectin (GNL lectin, Vector Laboratories, Burlingame, CA, USA).

    Techniques: Staining

    Comparison of oligomannosylated protein in mutant and Wt AB fish strains. Lectin blot and Coomassie blue-stained gels of embryo (48 hpf) ( A ), and tissues from adult strains ( C – J ). Quantification of the oligomannosylated proteins from embryos ( n = 5; Panel ( B )), and adult tissues, including swim bladder (SB, n = 3), skin (S, n = 3), heart (H, n = 3), skeletal muscle (SM, n = 3), spinal cord (SC, n = 3), brain (B, n = 4), brain regions 1 (R1, n = 5) and 2 (R2, n = 3) (Panel ( K )). Brain regions 1 includes olfactory bulb, telencephalon, habenula, and about half of optic tectum, while brain region 2 includes about half of the optic tectum, cerebellum, and medulla. Numbers adjacent to the blots signify protein markers (in kDa). Data were compared Student’s t-test (* p at 0.02).

    Journal: International journal of translational medicine (Basel, Switzerland)

    Article Title: Comparison of the Differing Impacts of Lowered N-Acetylglucosaminyltransferase-Ia/b Activity on Motor and Sensory Function in Zebrafish

    doi: 10.3390/ijtm5030036

    Figure Lengend Snippet: Comparison of oligomannosylated protein in mutant and Wt AB fish strains. Lectin blot and Coomassie blue-stained gels of embryo (48 hpf) ( A ), and tissues from adult strains ( C – J ). Quantification of the oligomannosylated proteins from embryos ( n = 5; Panel ( B )), and adult tissues, including swim bladder (SB, n = 3), skin (S, n = 3), heart (H, n = 3), skeletal muscle (SM, n = 3), spinal cord (SC, n = 3), brain (B, n = 4), brain regions 1 (R1, n = 5) and 2 (R2, n = 3) (Panel ( K )). Brain regions 1 includes olfactory bulb, telencephalon, habenula, and about half of optic tectum, while brain region 2 includes about half of the optic tectum, cerebellum, and medulla. Numbers adjacent to the blots signify protein markers (in kDa). Data were compared Student’s t-test (* p at 0.02).

    Article Snippet: The transferred proteins were then probed with Galanthus nivalis lectin (GNL lectin, Vector Laboratories, Burlingame, CA, USA).

    Techniques: Comparison, Mutagenesis, Staining